human normal cervical epithelial cells  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Thyrotropin-Releasing Hormone Gene Methylation as a Potential Biomarker for Anal Intraepithelial Neoplasia

doi: 10.3390/ijms262411784

Figure Lengend Snippet: TRH methylation analysis. ( A ) Data obtained from GSE186859 dataset, methylation levels at cg01009664 of TRH gene in anal cells with different anal lesions stratified by histology as normal (9 samples), AIN3 (13 samples) and anal cancer (121 samples). ( B ) Pyrosequencing analysis data of normal and cervical cancer cell lines.

Article Snippet: The DNA extracted from human cervical cancer cell lines containing HPV16, i.e., CaSki (ATCC CRL-1550, Manassas, VA, USA) and SiHa (ATCC HTB-35, Manassas, VA, USA), human cervical cancer cell line without HPV; C33A (ATCC HTB-31, Manassas, VA, USA), and normal human cervical epithelial cells, PCS480011 (ATCC PCS-480-011TM, Manassas, VA, USA) were used as a control for hypermethylated and hypomethylated TRH gene.

Techniques: Methylation

Journal: Hereditas

Article Title: circ-NOLC1 inhibits the development of cervical cancer by regulating miR-330-5p-PALM signaling axis.

doi: 10.1186/s41065-025-00478-5

Figure Lengend Snippet: Fig. 1 circ-NOLC1 was downregulated in CC cells. (A) The Gene Expression Omnibus dataset (accession no. GSE113696) analysis of differential circRNAs in CC cell lines (HeLa, CaSki, C-33 A, and SW756) compared to a normal cervical epithelial cell line (HcerEpic). Volcano map of differentially expressed genes (hsa_circ_0019686 is the most significantly downregulated gene in this dataset, adj.P.Val = 0.000246; logFC=-29.599789). RT-qPCR was used to detect the circ-NOLC1 expression level in subcellular components of (B) HeLa and (C) CaSki cells, including the nucleus (marker is U6) and cytoplasm (marker is GAPDH). The levels of circ-NOLC1 and linear NOLC1 in (D) HeLa and (E) CaSki cells treated with RNase R were detected by RT-qPCR. (F) RT-qPCR was ap plied to measure circ-NOLC1 expression in CC (HeLa, CaSki, C-33 A and SW756) and normal cervical epithelial (HcerEpic) cell lines. ***P < 0.001

Article Snippet: Cell lines and culture conditions Human CC [HeLa (CRM-CCL-2), CaSki (CRL-1550), C-33 A (HTB-31) and SW756 (CRL-3584)] and human normal cervical epithelial [HcerEpiC (PCS-480-011)] cell lines were obtained from the ATCC.

Techniques: Gene Expression, Quantitative RT-PCR, Expressing, Marker

Journal: Hereditas

Article Title: circ-NOLC1 inhibits the development of cervical cancer by regulating miR-330-5p-PALM signaling axis.

doi: 10.1186/s41065-025-00478-5

Figure Lengend Snippet: Fig. 4 circ-NOLC1 sponges miR-330-5p. (A) A Venn diagram illustrating the analysis of the circBank and starBase databases to predict circ-NOLC1 target miRNAs. (B) Binding sites between circ-NOLC1 and miR-330-5p were predicted using the starBase database. A dual luciferase reporter gene assay was conducted to elucidate the targeting relationship between circ-NOLC1 and miR-330-5p in (C) HeLa and (D) CaSki cells. RIP assay was performed to vali date the interaction between circ-NOLC1 and miR-330-5p in (E) HeLa and (F) CaSki cells. (G) The expression of miR-330-5p in CESC was predicted using the TCGA database (P = 1.62447832963153E-12). (H) RT-qPCR was employed to determine miR-330-5p expression in cervical cancer (HeLa and CaSki) and normal cervical epithelial (HcerEpic) cell lines. **P < 0.01, ***P < 0.001

Article Snippet: Cell lines and culture conditions Human CC [HeLa (CRM-CCL-2), CaSki (CRL-1550), C-33 A (HTB-31) and SW756 (CRL-3584)] and human normal cervical epithelial [HcerEpiC (PCS-480-011)] cell lines were obtained from the ATCC.

Techniques: Binding Assay, Luciferase, Reporter Gene Assay, Expressing, Quantitative RT-PCR

Journal: Hereditas

Article Title: circ-NOLC1 inhibits the development of cervical cancer by regulating miR-330-5p-PALM signaling axis.

doi: 10.1186/s41065-025-00478-5

Figure Lengend Snippet: Fig. 5 miR-330-5p interacts with PALM. (A) A Venn diagram illustrating the analysis of the TargetScanHuman 7.2 and miRDB databases to predict the target mRNAs of miR-330-5p. (B) The expression of PALM in CESC was predicted using the TCGA database (P = 2.14740003556813E-10). (C) RT-qPCR was employed to measure PALM expression in cervical cancer (HeLa and CaSki) and normal cervical epithelial (HcerEpic) cell lines. (D) Binding sites between miR-330-5p and PALM were predicted using the TargetScanHuman database. A dual luciferase reporter gene assay was conducted to elucidate the tar geting relationship between miR-330-5p and PALM in (E) HeLa and (F) CaSki cells. **P < 0.01, ***P < 0.001

Article Snippet: Cell lines and culture conditions Human CC [HeLa (CRM-CCL-2), CaSki (CRL-1550), C-33 A (HTB-31) and SW756 (CRL-3584)] and human normal cervical epithelial [HcerEpiC (PCS-480-011)] cell lines were obtained from the ATCC.

Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Luciferase, Reporter Gene Assay